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Manufacturer of Infectious Diseases Diagnostics


Hepatitis C can progress both with an acute and a chronic form, however, more than 50% of the infected individuals develop severe, life threatening chronic hepatitis with liver cirrhosis and hepatocellular carcinomas. 

Since the introduction of anti-HCV screening of blood donations in 1990, the incidence of this infection in transfusion recipients has been significantly reduced. The first generation of test showed limited sensitivity and specificity and were produced using recombinant proteins complementary to the NS4 region of the virus as antigens. Second generation tests, included recombinant / synthetic antigens from the Core and non-structural regions NS3 and NS4 which resulted in a remarkable improvement in sensitivity and specificity. The third generation tests include antigens from the NS5 region of the viral genome in addition to NS3, NS4 and the Core.

 Catalog No.
 WC-31S96 HCV-Ab ELISA Plus
 WJ-13 HCV-Ab Rapid Test  
 QZ-02 HCV-Ab Confirmation test  

Wantai HCV, Indirect ELISA (3rd Generation)

An indirect ELISA,a two-step incubation procedure. During incubation, HCV antibodies will be bound to the pre-coated HCV antigens. Anti-human IgG antibodies conjugated to the enzyme HRP are then added. These HRP-conjugated antibodies will bind to any antigen-antibody complexes. Chromogen solutions are added and the colourless Chromogens are hydrolyzed by the HRP to a blue-coloured product. The intensity of the colour is proportional to the amount of antibodies captured in the wells.

Wantai HCV, Sandwich ELISA (3rd Generation PLUS)

Wantai anti-HCV 3rd generation PLUS is based on the double antigen “sandwich” ELISA principle. This novel method for the testing of HCV antibodies allows detection of very early antibodies, including IgM and IgA in addition to the IgG which is the main target for detection of the previous generation assays. In addition, the method minimizes the non-specific reaction observed to occur with other methods and thus its utilization increases the specificity in detection.