The Hepatitis C (HCV) virus is an envelope, single-stranded positive sense RNA virus – a blood borne virus primarily transmitted by blood transfusion. Hepatitis C can take acute (asymptomatic) and chronic form, with more than 50% of infected individuals developing severe, life threatening chronic Hepatitis with liver cirrhosis and hepatocellular carcinomas. Globally, an estimated 71 million people have chronic Hepatitis C infection.

Wantai AiDTM anti-HCV ELISA is an indirect ELISA for detection of antibodies to HCV in a two-step incubation procedure. During incubation, HCV antibodies will be bound to the pre-coated HCV antigens. Anti-human IgG antibodies conjugated to the enzyme HRP are then added. These HRP-conjugated antibodies will bind to any antigen-antibody complexes. By adding chromogen solutions the colourless chromogens are hydrolyzed by the HRP to a blue-coloured product. The intensity of the colour is proportional to the amount of antibodies.

Wantai BioPharm’s AiDTM anti-HCV ELISA Plus is used for qualitative detection of antibodies to the Hepatitis C virus (HCV) in human serum or plasma. The kit features BIOTIN amplification resulting in increased sensitivity and specificity. The test is based on the double antigen “sandwich” ELISA principle. This novel method for the testing of HCV antibodies allows detection of very early antibodies, including IgM and IgA in addition to the IgG which is the main target for detection of the previous generation assays.

The Rapid Test for IgM antibody detection detects HEV-IgM antibodies and provides a clear test result within 10 minutes.

Wantai BioPharm confirmation tests are intended for use as supplemental tests for human serum or plasma specimens found to be repeatedly active in HCV and HIV antibody screening procedures. Tests used for the diagnosis of HIV and HCV infection require a high degree of both sensitivity and specificity, which is achieved using an algorithm combining two tests. Once antibodies have been detected by an initial ELISA test, a second supplemental test using the immunoblot procedure is applied and confirms or excludes the results of the initial ELISA test.